human recombinant dkk1  (R&D Systems)

Journal: Cancer Medicine

Article Title: Dickkopf 1 is expressed in normal fibroblasts during early stages of colorectal tumorigenesis

doi: 10.1002/cam4.6992

Figure Lengend Snippet: Identified 18 overexpressed genes.

Article Snippet: To analyze the function of DKK1 in colon cancer cell lines, HCT116 cells at a density of 4.0 × 10 3 cells per well were seeded in 96‐well plates and cultured in the medium supplemented with 200 ng/mL recombinant human DKK1 protein (R&D Systems, Minneapolis, MN, USA).

Techniques:

Journal: Cancer Medicine

Article Title: Dickkopf 1 is expressed in normal fibroblasts during early stages of colorectal tumorigenesis

doi: 10.1002/cam4.6992

Figure Lengend Snippet: Patient background of clinical specimens immunostained for DKK1.

Article Snippet: To analyze the function of DKK1 in colon cancer cell lines, HCT116 cells at a density of 4.0 × 10 3 cells per well were seeded in 96‐well plates and cultured in the medium supplemented with 200 ng/mL recombinant human DKK1 protein (R&D Systems, Minneapolis, MN, USA).

Techniques:

Journal: Cancer Medicine

Article Title: Dickkopf 1 is expressed in normal fibroblasts during early stages of colorectal tumorigenesis

doi: 10.1002/cam4.6992

Figure Lengend Snippet: Immunohistochemical staining of DKK1 in clinical specimens. (A) Representative images of DKK1 in human liver tissue, DKK2 in human hepatocarcinoma tissue, SFRP1 in human heart muscle tissue in positive control (scale bar, 1000 μm), normal epithelium (scale bar, 50 μm), nonadvanced adenoma (scale bar, 200 μm), advanced adenoma (scale bar, 500 μm), and advanced cancer (scale bar, 500 μm). (B) Comparison of specimens with immunohistochemical staining for αSMA and DKK1 in (a) nonadvanced adenoma and (b) advanced cancer. Scale bars: 100 μm. DKK1 , Dickkopf‐1; αSMA , α‐smooth muscle actin.

Article Snippet: To analyze the function of DKK1 in colon cancer cell lines, HCT116 cells at a density of 4.0 × 10 3 cells per well were seeded in 96‐well plates and cultured in the medium supplemented with 200 ng/mL recombinant human DKK1 protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Immunohistochemical staining, Staining, Positive Control, Comparison

Journal: Cancer Medicine

Article Title: Dickkopf 1 is expressed in normal fibroblasts during early stages of colorectal tumorigenesis

doi: 10.1002/cam4.6992

Figure Lengend Snippet: Histological expression of DKK1 in resection specimens.

Article Snippet: To analyze the function of DKK1 in colon cancer cell lines, HCT116 cells at a density of 4.0 × 10 3 cells per well were seeded in 96‐well plates and cultured in the medium supplemented with 200 ng/mL recombinant human DKK1 protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing

Journal: Cancer Medicine

Article Title: Dickkopf 1 is expressed in normal fibroblasts during early stages of colorectal tumorigenesis

doi: 10.1002/cam4.6992

Figure Lengend Snippet: Functional evaluation of DKK1 using human colon cancer cell line HCT116. (A) OD values obtained using the MTS assay after 24, 48, and 72 h of incubation of HCT116 cells with recombinant human DKK1 protein. n = 6. (B) Cell invasion assay after incubation of HCT116 cells with recombinant human DKK1 protein. n = 6. (C) Relative expression of DKK1 after siRNA transfection using Lipofectamine 3000. (D) MTS assay in HCT116 cells at 24, 48, and 72 h after siRNA transfection. n = 4. * p < 0.05; ** p < 0.01; *** p < 0.001. Rec DKK1 = recombinant human DKK1 protein.

Article Snippet: To analyze the function of DKK1 in colon cancer cell lines, HCT116 cells at a density of 4.0 × 10 3 cells per well were seeded in 96‐well plates and cultured in the medium supplemented with 200 ng/mL recombinant human DKK1 protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Functional Assay, MTS Assay, Incubation, Recombinant, Invasion Assay, Expressing, Transfection

Journal: Cancer Medicine

Article Title: Dickkopf 1 is expressed in normal fibroblasts during early stages of colorectal tumorigenesis

doi: 10.1002/cam4.6992

Figure Lengend Snippet: Serum DKK1 expression analysis. (A, B) ELISA of supernatant during co‐culture at Days 5–7; n = 2. Left: NFs monoculture, right: NFs co‐cultured with organoid (A) Using adenoma organoid. (B) Using cancer organoid. (C–F) Serum DKK1 expression in patients with colorectal tumors. (C) Comparison of serum DKK1 in samples obtained from patients with advanced cancer ( n = 32) and control ( n = 17). (D) ROC curve for (C), AUC = 0.78. (E) Comparison of serum DKK1 in paired serum samples from patients with advanced adenoma before and after treatment ( n = 17). (F) ROC curve for (E), AUC = 0.64. In (C) and (D), * p < 0.05; *** p < 0.001. AUC, area under the curve; DKK1 , Dickkopf‐1; post, post‐treatment; pre, pre‐treatment; ROC, receiver operating characteristic.

Article Snippet: To analyze the function of DKK1 in colon cancer cell lines, HCT116 cells at a density of 4.0 × 10 3 cells per well were seeded in 96‐well plates and cultured in the medium supplemented with 200 ng/mL recombinant human DKK1 protein (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Cell Culture, Comparison, Control